|
KMID : 0364820120480020087
|
|
Korean Journal of Microbiology
2012 Volume.48 No. 2 p.87 ~ p.92
|
|
|
Cellular Responses of Salmonella typhimurium Exposed to Green Tea Polyphenols
|
|
Choi Hyo-Kyung
Oh Kye-Heon
|
|
|
Abstract
|
|
|
The purpose of this study was to examine the cellular response of Salmonella typhimurium exposed to tea
polyphenols (TPP) extracted from Korean green tea (Camellia sinensis L.). TPP showed a dose-dependent
bactericidal effect on S. typhimurium. Analysis of cell membrane fatty acids of S. typhimurium cultures treated with
TPP identified unique changes in saturated and unsaturated fatty acids, while scanning electron microscopic analysis
demonstrated the presence of perforations and irregular rod forms with wrinkled surfaces in cells treated with TPP.
Two-dimensional polyacrylamide gel electrophoresis of soluble protein fractions from S. typhimurium cultures
showed 16 protein spots increased by TPP. These up-regulated proteins including proteins involved in antioxidants
and chaperons, transcript and binding proteins, energy and DNA metabolism were identified by peptide mass
fingerprinting using MALDI-TOF. These results provide clues for understanding the mechanism of TPP induced
stress and cytotoxicity on S. typhimurium.
|
|
|
KEYWORD
|
|
|
cellular responses, green tea polyphenols, proteomic analysis, TPP
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|